Review



primescripttm rt master mix kit  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    TaKaRa primescripttm rt master mix kit
    Primescripttm Rt Master Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26665 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+master+mix/PrimeScript+RT+Master+Mix/pmc13035274-172-7-15
    Average 99 stars, based on 26665 article reviews
    primescripttm rt master mix kit - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Reverse Transcription:

    Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
    Article Snippet: Total RNA was extracted from cultured cells and peri-wound tissue samples using the RNeasy Mini Kit (Qiagen, Cat# 74014), following the manufacturer's protocol. .. Reverse transcription was performed using the PrimeScriptTM RT Master Mix (Takara, Cat# RR036A). .. Quantitative real-time PCR (qPCR) was conducted using SYBR Green chemistry on an ABI 7500 Real-Time PCR System (Applied Biosystems).

    Article Title: Gestational progesterone restores menstrual cycle in PCOS patients via enhancing ovary estrogen production
    Article Snippet: Frozen tissues were homogenized in 1 mL TRIzol (Invitrogen) with a tissue homogenizer. .. Total RNA was isolated as described in the manufacturer’s instructions and then subjected to reverse transcription with PrimeScriptTM RT Master Mix (Takara). ..

    Article Title: Nanozyme hydrogels remodel pathological microenvironment for temporomandibular joint osteoarthritis therapy via inhibiting MAPK signal pathway
    Article Snippet: .. RNA was reverse transcribed to cDNA (Complementary DNA) using a real-time PCR machine (VeritiProTM Thermal Cycler, 96-well) and the reverse transcription cDNA synthesis kit (PrimeScriptTM RT Master Mix, Takara). .. Gene expression was analyzed using a real-time PCR instrument (QuantStudioTM 1 Plus, Thermo Fisher Scientific) using highly specific qPCR reagents (TB Green® Premix Ex TaqTM II, Takara), as instructed.

    Article Title: Inhibition of miR-33b-5p promotes osteoblast differentiation by regulating ABCA1 in cellular and animal models of osteoporosis.
    Article Snippet: Total RNA was extracted from BMSCs using TRIzol reagent (Invitrogen) immediately after DEX treatment or plasmid transfection. .. The collected RNA was reverse transcribed into cDNA using PrimeScriptTM RT Master Mix (Takara, Dalian, China) for mRNA detection or a miRNA first-strand cDNA synthesis kit (Sangon, Shanghai, China) for miRNA detection. .. PCR was performed using a SYBR® Premix Ex TaqTM II kit (Takara) on an ABI 7500 RealTime PCR System (California, USA).

    Synthesized:

    Article Title: Patient-derived AMOTL1 mutations lead to defective cell migration and tissue development
    Article Snippet: Total mRNA was extracted and purified using TAKARA MiniBEST Universal RNA Extraction Kit following the manufacturer’s instructions. .. The cDNA was synthesized using TAKARA PrimeScriptTM RT Master Mix. .. RT-PCR was carried out using ABI 7500 FAST instrument and TAKARA SYBR Premix Ex Taq (Tli RNaseH Plus) kit.

    Article Title: An N, S-acetylated L-cysteine-cysteamine conjugate hinders pyocyanin redox cycling to weaken Pseudomonas aeruginosa biofilm and dampens LPS-driven acute pulmonary inflammation
    Article Snippet: Total RNA was extracted using the RNeasy Plus mini kit (Qiagen, Germany) after lysing the cells with RLT buffer supplemented with 1% βmercaptoethanol (Sigma, Germany). .. According to the manufacturer’s instructions, cDNA was synthesized using Takara PrimeScriptTM RT Master Mix (Takara, Japan) from 250 ng of total RNA. .. The RT-PCR reactions were performed on a QuantStudio 5 (Applied Biosystems, USA) in triplicate using PowerUp® SYBR Green Mastermix (Applied Biosystems, USA).

    Article Title: DAPT Mitigates Osteoarthritic Cartilage Degeneration and Enhances Articular Repair Through Targeted Modulation of the Gucy1a3 and Notch Signaling Axis
    Article Snippet: .. Total RNA was extracted using TRIzol ® reagent (Takara), and cDNA was synthesized using the PrimeScriptTM RT Master Mix (Takara). ..

    Isolation:

    Article Title: Gestational progesterone restores menstrual cycle in PCOS patients via enhancing ovary estrogen production
    Article Snippet: Frozen tissues were homogenized in 1 mL TRIzol (Invitrogen) with a tissue homogenizer. .. Total RNA was isolated as described in the manufacturer’s instructions and then subjected to reverse transcription with PrimeScriptTM RT Master Mix (Takara). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Nanozyme hydrogels remodel pathological microenvironment for temporomandibular joint osteoarthritis therapy via inhibiting MAPK signal pathway
    Article Snippet: .. RNA was reverse transcribed to cDNA (Complementary DNA) using a real-time PCR machine (VeritiProTM Thermal Cycler, 96-well) and the reverse transcription cDNA synthesis kit (PrimeScriptTM RT Master Mix, Takara). .. Gene expression was analyzed using a real-time PCR instrument (QuantStudioTM 1 Plus, Thermo Fisher Scientific) using highly specific qPCR reagents (TB Green® Premix Ex TaqTM II, Takara), as instructed.

    cDNA Synthesis:

    Article Title: Nanozyme hydrogels remodel pathological microenvironment for temporomandibular joint osteoarthritis therapy via inhibiting MAPK signal pathway
    Article Snippet: .. RNA was reverse transcribed to cDNA (Complementary DNA) using a real-time PCR machine (VeritiProTM Thermal Cycler, 96-well) and the reverse transcription cDNA synthesis kit (PrimeScriptTM RT Master Mix, Takara). .. Gene expression was analyzed using a real-time PCR instrument (QuantStudioTM 1 Plus, Thermo Fisher Scientific) using highly specific qPCR reagents (TB Green® Premix Ex TaqTM II, Takara), as instructed.

    Article Title: Inhibition of miR-33b-5p promotes osteoblast differentiation by regulating ABCA1 in cellular and animal models of osteoporosis.
    Article Snippet: Total RNA was extracted from BMSCs using TRIzol reagent (Invitrogen) immediately after DEX treatment or plasmid transfection. .. The collected RNA was reverse transcribed into cDNA using PrimeScriptTM RT Master Mix (Takara, Dalian, China) for mRNA detection or a miRNA first-strand cDNA synthesis kit (Sangon, Shanghai, China) for miRNA detection. .. PCR was performed using a SYBR® Premix Ex TaqTM II kit (Takara) on an ABI 7500 RealTime PCR System (California, USA).



    Similar Products

    99
    TaKaRa primescripttm rt master mix kit
    Primescripttm Rt Master Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+master+mix/PrimeScript+RT+Master+Mix/pmc13035274-172-7-15
    Average 99 stars, based on 1 article reviews
    primescripttm rt master mix kit - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa primescripttm rt master mix
    Primescripttm Rt Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+master+mix/PrimeScript+RT+Master+Mix/pmc13049664-179-6-10
    Average 99 stars, based on 1 article reviews
    primescripttm rt master mix - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa primescripttm rt mastermix
    Primescripttm Rt Mastermix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+master+mix/PrimeScript+RT+Master+Mix/pmc13042270-65-10-13
    Average 99 stars, based on 1 article reviews
    primescripttm rt mastermix - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    Image Search Results